1. Preparing nutrient agar media, autoclaving and pouring plates for soil bacteria assay.

-weigh dry media use distilled water

-autoclave media in flasks

-autoclave spatulas and tubes for soil samples

-meat thermometer for soil temperatures

-check Mars sites for Viking data on soil experiments

2. Transferring and culturing prokaryotes.

3. Phenol Red-Lactose broth tests

-metabolism tests